Green Qpcr Primers

The azuraquant green fast qpcr mix is a ready to use 2x master mix for use in real time quantitative pcr assays in which intercalating dye based detection provides the option of a post amplification melt profile.

Green qpcr primers.

The kit also includes a control primer set designed for amplification of the synthetic rna spike in provided in the mircury lna rt kit. Sybr green for qpcr. Azuraquant green and probe fast qpcr master mixes for real time pcr real time pcr primer enzymes. General description overview compatible with any thermal cycler kicqstart primers are ready to order pre designed primer pairs for quantifying gene expression by sybr green i rt qpcr reverse transcription quantitative real time pcr.

Use of other reagents for qpcr will affect the results. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. Our primer collection covers the entire human and mouse genomes. The mircury lna mirna pcr assays and panels have been validated using the mircury sybr green master mix.

The primers are meticulously designed using origene s proprietary primer design algorithm developed from 10 000 qpcr experiments. Design sybr green qpcr primers experimental protocol oct 31 2013 recommendations. To comply with miqe they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off target amplification 1. 1 find the ncbi gene.

Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases.