Highly inclined and laminated optical sheet hilo microscopy is an optical technique that employs a highly inclined laser beam to illuminate the sample with a thin sheet of light that can be scanned through the sample volume 1 hilo is an efficient illumination technique when applied to fluorescence imaging of thick samples owing to the confined illumination volume that allows high. Nature methods 5 159 161. In this study we developed simple easy to use microfluidic device for highly inclined and laminated optical sheet hilo microscopy using a model plant physcomitrella patens.
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